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Cells and tissues in culture: methods, biology, and physiology
Edward Nevill Willmer
Visualizzazione frammento - 1965
acid alcohol amino-acids Anat animal cells antibiotics apparatus aseptic room autoclave balanced salt solution Biochem Biol blood Cancer Inst Cell Res cell strains cell suspension cells in tissue cells in vitro cent centrifuge chamber chick embryo chick heart chromosomes clean concentration containing cultivation cultured cells cystine described developed dilute distilled water Earle effect embryo embryo extract epithelial essential explants fibroblasts filters fluid glass glassware glucose glutamine grow grown growth growth medium incubator laboratory large numbers mammalian cells material medium metabolism method minutes mitosis mouse normal nutrition obtained organ culture particularly Pasteur pipette Petri dish Physiol pipette placed plant tissue culture plasma plasma clot poliomyelitis prepared Proc procedure protein removed Rinse rubber serum sodium sterile sterilised stock solution stoppers studies survival technique temperature test-tubes tion tissue cells tissue culture toxic transferred trypsin trypsinisation usually vessel virus viruses vitamin vitro washed