Laboratory Procedures in Clinical MicrobiologyJ.A. Washington Springer New York, 6 mar 1981 - 856 pagine Although there are a number of comprehensive books in clinical micro biology, there remains a need for a manual that can be used in the clinical laboratory to guide the daily performance of its work. Most of the existing publications provide detailed and precise information, for example, by which a microorganism can be characterized and identified beyond any doubt; however, the number of tests involved in this process exceeds the capabilities and resources of most clinical laboratories and are irrelevant for patient care. It is, therefore, necessary in any clinical laboratory to extract from reference manuals, textbooks, and journals those tests and procedures that are to be used to complete the daily workload !is efficiently and accurately as possible. It is also essential in the clinical laboratory to determine, on the basis of the kind of specimen being examined, which microorganisms are clinically relevant and require isolation and identifica tion and which should either be excluded selectively or simply regarded as indigenous Hora and, therefore, not specifically identified. Cost and time limit a laboratory's resources, and priorities must be established for handling the workload. The procedures described in this manual are those selected by our staff for use in the clinical laboratory on the basis of clinical relevance, accuracy, reproducibility, and efficiency. ' Alternative procedures, when considered equivalent on the basis of personal or published experience, have been in cluded where appropriate. |
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Collection and Handling of Specimens | 1 |
Specific Guidelines for Specimen Collection | 24 |
References | 66 |
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acid aerobic anaerobic bacteria antibiotic antibody antigen antimicrobial antiserum assay Bacteroides blood agar bottle broth buffer Candida Candida albicans carbohydrate catalase cell cultures Chlamydia Clin clinical cocci color concentration containing Cryptococcus Cryptococcus neoformans cysts detect diagnosis Difco Laboratories differential dilution disease disk Distilled water Distilled water Final echovirus Enterobacteriaceae Escherichia coli examined fermentation Figure fluid fluorescence fragilis fungi gentamicin glucose gram-negative gram-negative bacilli gram-positive GRBCs growth Haemophilus hyphae identification incubation infection influenza inhibition inoculated inoculum Isolated colonies medium method Microbiol microscopic morphology mycobacteria Mycoplasma NaCl Negative test Neisseria nitrate organisms patients penicillin petri dish pneumoniae Positive test prepared Procedure produce Pseudomonas Pseudomonas aeruginosa Reagents respiratory serological serum slant slide smears Sodium solution species specimen stain Sterilize at 121°C Streptococcus subculture susceptibility testing suspension swab Table tion tissue titer tobramycin urease urine usually vial viral virus viruses water Final pH yeasts µg/ml