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Molecular cloning:

a laboratory manual, Volume 2
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Cold Spring Harbor Laboratory, 1989

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Indice

TEST LIGATION 3 29
8-3
SYNTHESIS OF THE FIRST STRAND OF cDNA 8
8-11
COMMONLY USED BUFFERS B
8-20
Copyright

85 sezioni non visualizzate

Altre edizioni - Visualizza tutto

Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,David William Russell
Anteprima limitata - 2001
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,E. F. Fritsch,Tom Maniatis
Anteprima non disponibile - 1989
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual
Joseph Sambrook,Edward F. Fritsch,Thomas Maniatis
Anteprima non disponibile - 1989
Visualizza tutto »

Parole e frasi comuni

agarose gel aliquots amino acid amplification antibody appropriate bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography Cl pH cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing deletions denatured described digestion dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease formamide gel electrophoresis gene genomic hybridization Incubate the reaction inserted Klenow fragment ligation linear linkers mammalian method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filter nucleic acids nucleotides oligonucleotide phagemid pipette plaques plasmid plasmid DNA plates polyacrylamide prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature sample screening single-stranded DNA solution specific activity step Store strand of cDNA synthesis T4 DNA ligase target DNA target sequence termini transcription transfer volume

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Informazioni bibliografiche

QR code for Molecular cloning
TitoloMolecular cloning: a laboratory manual, Volume 2
Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091
AutoriJoseph Sambrook, E. F. Fritsch, Tom Maniatis
Edizione2
EditoreCold Spring Harbor Laboratory, 1989
Provenienza dell'originalela University of Michigan
Digitalizzato15 lug 2008
ISBN0879693096, 9780879693091
  
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