Methods in Aquatic Microbiology

6H₂O added aerobic agar ammonia amount anaerobes apparatus aquatic autoclave Azotobacter beef-peptone boiled bottle broth CaCl2 carbon cells cellulose colonies color compounds concentration count cover glass decomposition determined dilution Distilled water Distilled water 1000 dried drop electrode ethyl alcohol filter paper filtration flask fluorescent fluorochromes g Agar g MgSO4 g NaCl gelatin Glucose growth hydrogen incubator inoculation inoculum iron bacteria isolation isotopes K₂HPO4 layer liquid Medium Distilled water membrane filter method methylene blue MgSO MgSO4 7H₂O microbiology microorganisms microscope milliliters minutes mixture nitrates nitrogen nutrient medium obtained organic oxidation oxygen Peptone petri dish pipette placed plates poured into test precipitate prepared pure cultures purple sulfur bacteria radioactive reagent rinsed sediment slides smear sodium solution species specimen spores Staining sterile stopper sulfate sulfur bacteria sulfuric acid surface Tap water temperature test tubes tion washed water masses water sample Winogradsky Yeast extract