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Molecular cloning:

a laboratory manual, Volume 1
Copertina anteriore
1 Recensione
Cold Spring Harbor Laboratory, 1989 - 3 pagine

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Indice

Replication and Incompatibility 1
1-3
Constructing Expression Libraries in Plasmid and Bacteriophage
1-12
Oligonucleotidemediated Mutagenesis 15 51
1-15
Copyright

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Altre edizioni - Visualizza tutto

Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,David William Russell
Anteprima limitata - 2001
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,E. F. Fritsch,Tom Maniatis
Anteprima non disponibile - 1989
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual
Joseph Sambrook,Edward F. Fritsch,Thomas Maniatis
Anteprima non disponibile - 1989
Visualizza tutto »

Parole e frasi comuni

agar plate agarose gel aliquots ampicillin antibiotic bacteriophage particles bacteriophage T4 DNA BamHI buffer carry cDNA cells centrifugation at 12,000g Cl pH cleaved cloning coli containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic exonuclease extracts final concentration foreign DNA formamide gel electrophoresis gene genetic gradients Hindlll host hours at 37°C hybridization Incubate infected inserted ligation reaction linear lysogenic method microfuge tube minutes at 4°C minutes at room mixture mutations nitrocellulose nitrocellulose filter Nucleic Acids nucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified recA recombinant remove replication restriction enzymes RNAase room temperature segment of foreign sequences single-stranded DNA solution step sterile stored strains strand supernatant T4 DNA ligase teriophage transfer transformation vector DNA vitro volume

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Informazioni sull'autore (1989)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

Informazioni bibliografiche

QR code for Molecular cloning
TitoloMolecular cloning: a laboratory manual, Volume 1
Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091
AutoriJoseph Sambrook, E. F. Fritsch, Tom Maniatis
fotografie diE. F. Fritsch
Edizione2, illustrata
EditoreCold Spring Harbor Laboratory, 1989
Provenienza dell'originalela University of Michigan
Digitalizzato23 lug 2008
ISBN0879693096, 9780879693091
Lunghezza3 pagine
  
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