Molecular Cloning: A Laboratory Manual, Volume 1Cold Spring Harbor Laboratory, 1989 - 676 pagine |
Dall'interno del libro
Risultati 1-3 di 60
Pagina 2-60
... sterile rich medium ( e.g. , NZCYM or LB ) supplemented with 0.2 % maltose in a sterile 250 - ml flask and inoculate with a single bacterial colony . Grow the culture overnight at 37 ° C with moderate agitation ( 250 cycles / minute in ...
... sterile rich medium ( e.g. , NZCYM or LB ) supplemented with 0.2 % maltose in a sterile 250 - ml flask and inoculate with a single bacterial colony . Grow the culture overnight at 37 ° C with moderate agitation ( 250 cycles / minute in ...
Pagina 4-24
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
Pagina 7-35
... sterile water , and the solutions should then be treated overnight with 0.1 % diethyl pyrocarbonate ( DEPC ) at 37 ° C and heated to 100 ° C for 15 minutes . After the sucrose solutions have cooled to room temperature , sterile ...
... sterile water , and the solutions should then be treated overnight with 0.1 % diethyl pyrocarbonate ( DEPC ) at 37 ° C and heated to 100 ° C for 15 minutes . After the sucrose solutions have cooled to room temperature , sterile ...
Sommario
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Altre edizioni - Visualizza tutto
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Parole e frasi comuni
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml