Molecular Cloning: A Laboratory Manual, Volumi 1-3Cold Spring Harbor Laboratory Press, 1989 - 1659 pagine Construction and Analysis of cDNA Libraries; Analysis and Cloning of Eukaryotic Genomic DNA; Preparation of Radiolabeled DNA and RNA Probes;Synthetic Oligonucleotic Probes; Screening Expression Libraries with Antibodies and Oligonucleotides;DNA Sequencing; In Vitro Amplification of DNA by the Polymerase Chain Reaction; Site-directed Mutagenesis of Cloned DNA. |