Cosa dicono le persone - Scrivi una recensioneReview: Bioconjugate TechniquesRecensione dell'utente - Rafe - GoodreadsA fabulously useful book for the chemical biologist. Presents straightforward coupling and labeling strategies with detailed protocols. Leggi recensione completa Libri correlati
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Parole e frasi comuni0.1 M sodium activity addition aldehyde amide bond amine groups amine-containing molecules amine-reactive anhydride antibody antigen aqueous solution assay avidin azide binding bioconjugation biotin biotinylated buffer carbodiimide carbohydrate carbon cells Chapter chelating complex compounds concentration conjugation conjugation reaction containing coupling covalent create crosslinking dendrimer derivative derivatization detection Dissolve disulfide DMSO enzyme ester Figure fluorescent following protocol functional groups gel filtration glycans hapten heterobifunctional homobifunctional hours at room hydrazide hydrolysis hydrophilic hydrophobic hydroxyl interactions labeled ligands linkages liposomes macromolecules maleimide maleimide group mg/ml mixing modification molar excess molecules NaCl NHS ester nucleophiles organic solvent oxidation particles peptide phenyl azide polymer primary amine probes protein Purify react reactive groups reagent residues result ring room temperature Section sodium periodate sodium phosphate solubility spacer SPDP stable stock solution strept)avidin structure sulfhydryl groups sulfhydryl-containing sulfhydryl-reactive sulfo-NHS surface Thermo Fisher thioether thiol thiolated tion toxin Brani popolariPagina 1061 - A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Pagina 1063 - Friden, PM, Walus, LR, Watson, P., Doctrow, SR, Kozarich, JW, Backman, C., Bergman, H., Hoffer, B., Bloom, F., and Granholm, A.-C, Blood-brain barrier penetration and in vivo activity of an NGF conjugate. Pagina 1085 - Langer, PR, Waldrop, AA, and Ward, DC (1981) Enzymatic synthesis of biotinlabeled polynucleotides: novel nucleic acid affinity probes. Pagina 1074 - I. (1968) Diphtheria toxin-dependent adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis. J. Biol. Chem. 243, 3553-3555. Pagina 1077 - Jaiswal JK, Mattoussi H, Mauro JM, Simon SM. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Pagina 1051 - Butler, JE, Ni, L., Nessler, R., Joshi, KS, Suter, M., Rosenberg, B., Chang, J., Brown, WR, and Cantarero, LA (1992) The physical and functional behavior of capture antibodies adsorbed on polystyrene. Pagina 1062 - Fields, S. and Song, O. (1989) A novel genetic system to detect protein-protein interactions. Nature 340, 245-246. Pagina 1046 - A novel method of activation of cross-linked agaroses with l,l'-carbonyldiimidazole which gives a matrix for affinity chromatography devoid of additional charged groups. J. Biol. Chem. 254, 2572-2574. Riferimenti a questo libroDa altri libri
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