General Procedures for Primary Cell Culture1980 - 7 pagine |
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Prova ad ampliare la ricerca a tutti i volumi: sporophyte
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antibiotics and antimycotic antimycotic agents bone marrow buffy coat cannula and discard cell population cell suspension cells in culture cellular debris chelating agents Cloning Cylinder Collagenase collected colonies contain coverslip fragments culture medium culture vessel cytoplasmic Dilution Plating dissociation methods enzymatic enzymes or chelating Epithelial-like cells etched X's explants are placed Fibroblastic figure forceps fresh enzyme solution Glass chips glass fragments growth medium heparin incubated Inferior Vena Cava kanamycin Kidney Laboratory animals Leukocytes liver Macrophages minced tissue mm³ morphological Multiple Well Plate niques Organ Culture penicillin Penicylinders Petri dishes prevent the explant primary cell culture primary culture pronase RBC's red blood cells removal of tissue Remove the flask rinse salt and/or enzyme salt solution scalpels scissors serum quality single cell soft agar specific cell types sterile streptomycin subcultured sulfate supernatant syringe Tissue and Organ tissue culture dish tissue fragments tissue with enzymes toxic trypsin ture viable cells VIVO Perfusion