The Condensed Protocols from Molecular Cloning: A Laboratory Manual

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CSHL Press, 2006 - 800 pagine
The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
 

Sommario

TitleAuthors
77
Ch 3 Working with Bacteriophage M13 Vectors
133
Ch 4 Working with Highcapacity Vectors
153
Ch 5 Gel Electrophoresis of DNA and Pulsedfield Agarose Gel Electrophoresis
187
Ch 6 Preparation and Analysis of Eukaryotic Genomic DNA
237
Ch 7 Extraction Purification and Analysis of mRNA from Eukaryotic Cells
269
Ch 8 In Vitro Amplification of DNA by PCR
309
Ch 9 Preparation of Radiolabeled DNA and RNA Probes
357
Ch 15 Expression of Cloned Genes in E coli
573
Ch 16 Introducing Cloned Genes into Cultured Mammalian Cells
599
Ch17 Analysis of Gene Expression in Mammalian Cells
621
Ch 18 Protein Interaction Technologies
655
Appendices Index
689
Appendix 1 Preparation of Reagents and Buffers Used in Molecular Cloning
691
Appendix 2 Media
717
Appendix 3 Commonly Used Techniques in Molecular Cloning
727

Ch 10 Working with Synthetic Oligonucleotide Probes
401
Ch 11 Preparation of cDNA Libraries and Gene Identification
427
Ch 12 DNA Sequencing
467
Ch 13 Mutagenesis
505
Ch 14 Screening Expression Libraries
543
Appendix 4 Cautions
769
Index
779
Back Cover
801
Copyright

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