GB 5009.287-2022 Translated English of Chinese Standard (GB5009.287-2022): National food safety standard - Determination of bixinin foodshttps://www.chinesestandard.net, 21 ago 2022 - 10 pagine This Standard specifies the method of liquid chromatography for the determination of bixin (bixin and norbixin) in foods. This Standard is applicable to the determination of bixin and norbixin in cheese, processed cheese and similar products, margarine and similar products, non-dairy creamers, frozen drinks, jams, chocolate and chocolate products, candies, grains and grain products, baked goods, western-style ham, meat sausages, compound seasonings, beverages, jelly and puffed food. |
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50 mL plastic accurate to 0.01 Add 20 mL ammonia ethanol solution ammonia water Analytical anhydrous ethanol Appendix BHT-ethanol solution 3.2.2 BHT-petroleum ether solution bixin or norbixin bixin solution containing brown volumetric flask certified chromatographic column chromatographically pure cis-bixin standard solutions cis-norbixin and cis-bixin constant volume Detection determination of bixin diatomaceous earth dichloromethane dilute discard the upper dissolve ethanol solution 3.2.4 expressed extract Figure A.1 Food g accurate g of BHT glacial acetic acid inject layer of solution liquid chromatogram mass concentration measure-take mg/kg mg/L mixed standard mL brown volumetric mL plastic centrifuge nitrogen concentration device norbixin and bixin obtained peak area perform vortex oscillation petroleum ether plastic centrifuge tube Preparation purification r/min reach a constant Reagents reference substance reference substance certificate Reserve retention samples Weigh-take 0.5 solution containing cis specified specimen solution standard curve standard stock solution standard working solution stopper trans-configurations Transfer upper layer weigh-take 0.5 g