Laboratory methods in histotechnology
American Registry of Pathology, 1992 - 279 pagine
This protocol laboratory manual is designed for daily laboratory use. With vivid color photographs, descriptive line art & detailed special staining techniques, histotechnologists can achieve the highest quality preparations. Written by experienced histotechnologists & pathologists at the AFIP, this up-to-date comprehensive laboratory manual gives histotechnologists detailed information & helpful tips on how to produce optimal slides. Now available from: The American Registry of Pathology AFIP, Room 1077, Washington, D.C. 20306-600, (202) 576-2940. Cost: $35.00, 25 percent discount to U.S. bookstores. $3.00 S&H per book, domestic address. 25 percent S&H per book, foreign addresses.
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100 ml distilled 15 minutes 30 minutes absolute ethyl alcohol ACID SOLUTION Ch AFIP alcian blue ammonium hydroxide Basic fuchsin block blue solution buffer solution buffered neutral formalin celloidin cells changes of distilled clear through 95 Counterstain cresyl cytoplasm Dehydrate and clear Deparaffinize and hydrate Distilled water 100.0 embedding eosin ethyl alcohol fast red solution ferric chloride fibers filter FIXATION fuchsin solution gelatin glacial acetic acid gm sodium histotechnology hydrate slides hydrate to distilled hydrochloric acid knife laboratory lithium carbonate MAYER'S HEMATOXYLIN SOLUTION METHOD micrometers microtome ml distilled water ml PROCEDURE Mount with resinous nuclear fast red Nuclei oxide Paraffin Pararosaniline phosphotungstic acid picric acid processing resinous medium RESULTS See Fig Rinse in distilled running tap water running water silver nitrate silver nitrate solution sodium hydroxide sodium iodate sodium phosphate SODIUM THIOSULFATE solution for 30 special stains specimens sulfate tap water tissue Wash in running xylene