YY/T 1497-2016 Translated English of Chinese Standard. (YYT 1497-2016, YY/T1497-2016, YYT1497-2016): Evaluation Test Method for the Viral Filtration Efficiency (VFE) of Medical Protective Face Mask Materials - Test Method Using Phi-X174 Bacteriophagehttps://www.chinesestandard.net, 31 mar 2020 - 13 pagine This Standard stipulates the method of using Phi-X174 bacteriophage suspension liquid as a surrogate microbe to test the viral filtration efficiency (VFE) of medical protective face masks or face mask materials. This Standard is applicable to medical protective face masks or face mask materials which have requirements for the evaluation of the viral filtration efficiency (VFE). |
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agar culture medium AGI-30 liquid impact bacteriophage nutrient broth Bacteriophage refers bacteriophage solution bacteriophage suspension liquid Beijing Calcium chloride 0.2 challenging suspension liquid chloride 0.2 g chloride 5 g concentration of bacteriophage conducting pressure steam conical flask corresponding dilution factor cultivate culture solution document Escherichia coli expressed in PFU face mask materials Filtration Efficiency VFE g Add water g Calcium g Calcium chloride g Potassium g Potassium chloride GB/T host cells infection and lysis Inoculating loop L/min layer of agar liquid impact samplers maintain the temperature medical protective face number of plaques PFU/mL pH value plaque count plates of test positive control group Potassium chloride pressure steam sterilization protective face masks r/min rotating speed sample group specified date Standard sterile peptone water sterilization for 20 supernatant temperature of 45 Test equipment test method test sample test solution upper layer value is 7.3 value of plaque viral filtration efficiency virus