Standard operating procedure for soil enzyme activities: β-glucosidases, arylsulfatase, N-acetyl-β-glucosaminidase, dehydrogenase, phosphomonoesterasesFood & Agriculture Org., 24 feb 2025 - 78 pagine his standard operating procedure (SOP) has been harmonized by the joint working group of the FAO's Global Soil Laboratory Network (GLOSOLAN) and the International Network on Soil Biodiversity (NETSOB). The protocol focuses on the determination of five soil enzyme activities: β-Glucosidases, Arylsulfatase, N-acetyl-β-Glucosaminidase, Dehydrogenase, and Phosphomonoesterases. After a general introduction on the importance of soil enzyme as key indicator for soil health and few remarks on the sampling strategies, the determination of each enzyme is presented separately with step-by-step instructions. |
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50 ml Erlenmeyer Absorbance nm absorbance readings Acosta-Martinez activity in soil analysis Biochemistry Biology and Biochemistry CaCl2 Calcium chloride calculation to obtain calibration curve Calibration graph Chaer chemical chloride Daughtridge dehydrogenase activity determination of PNP Dissolve Eivazi and Tabatabai environmental enzyme assay Erlenmeyer flasks Example of calculation filter paper filtrate is calculated graph and regression hydrolysis Karlen Laboratory control sample Lopes Lorenz and Dick Margenot Mendes methanol microbial indicators ml Erlenmeyer flasks Nakayama NaOH NCBI obtain final results organic matter Oxisols percent performed phosphate pipette PNP content PNP released PNP/g PNPP Precision test Procedure protocol PubChem regression equation Reis Junior Sample preparation SH assessments Sodium hydroxide Soil Biology soil enzyme activities Soil Health soil is incubated soil organic soil quality soil replicate Soil Science soil suspension soil/h spectrophotometer SSSA standard PNP solution standard solution stopper storage substrate Tabatabai toluene TPF concentration TPF/ml volumetric flask µg/ml