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Pagina 81
Culture Size If the culture exceeds a certain size the central cells die , either from
lack of oxygen or glucose , or from poisoning by accumulated CO2 , lactic acid or
other waste product . The diffusion rate of one or other of these substances ...
Culture Size If the culture exceeds a certain size the central cells die , either from
lack of oxygen or glucose , or from poisoning by accumulated CO2 , lactic acid or
other waste product . The diffusion rate of one or other of these substances ...
Pagina 82
This procedure did improve oxygenation of the basal part of the culture and
slightly larger cultures could be used , but it had no other advantage and it was
not adopted for routine use . By what other means could oxygen supply be
improved ?
This procedure did improve oxygenation of the basal part of the culture and
slightly larger cultures could be used , but it had no other advantage and it was
not adopted for routine use . By what other means could oxygen supply be
improved ?
Pagina 260
Effect of Oxygen Tension Analogous to the Crabtree effect is the Pasteur effect .
When the oxygen tension in the medium is lowered , glucose utilization has been
found by some workers ( Danes et al . , 1963 ; Suschny et al . , 1958 ) to increase
...
Effect of Oxygen Tension Analogous to the Crabtree effect is the Pasteur effect .
When the oxygen tension in the medium is lowered , glucose utilization has been
found by some workers ( Danes et al . , 1963 ; Suschny et al . , 1958 ) to increase
...
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Sommario
12 | 288 |
Deoxyribonucleic Acid and Ribonucleic Acid Synthesis | 317 |
Mucopolysaccharide Metabolism in Tissue Culture | 353 |
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Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 Edward Nevill Willmer Visualizzazione frammento - 1965 |
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action activity aggregates amino acids amount animal appear become Biochem Biol biophys bone Cancer cell cultures Cell Res cellular changes chemical chick chromosomes complete concentration containing cultivation cytoplasm defined dependent determination differentiation direct dissociated division Earle effect embryo enzymes essential evidence experiments explants extract fact factors fibroblasts fluid formation function gland glass glucose growing grown growth hormones human important increase induced influence inhibition isolated known less mammalian material mechanism medium metabolism method mitosis Moscona mouse movement mucopolysaccharides Nature normal nutritional observed obtained occur organ organ culture original oxygen period plasma population possible present probably problems Proc produced properties protein relation requirements response serum showed shown similar skin solution specific stage strain structure studies substances suggested surface synthesis synthetic tissue culture types various vitamin vitro
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