Methods in Avian EmbryologyAcademic Press, 16 mag 1996 - 369 pagine A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer. Key Features* Complete and easy-to-follow procedures* Helpful illustrations* Distinguished group of authors* Wide range of approaches |
Sommario
| 23 | |
| 61 | |
Chapter 4 Manipulation of the Avian Segmental Plate in Vivo | 81 |
An Embryo Fillet Preparation | 93 |
Chapter 6 Embryo Slices | 109 |
Chapter 7 Operations on Limb Buds of Avian Embryos | 125 |
Chapter 8 Iontophoretic Dye Labeling of Embryonic Cells | 147 |
Chapter 9 Gene Transfer Using ReplicationDefective Retroviral and Adenoviral Vectors | 161 |
Chapter 12 Micromass Cultures of Limb and Other Mesenchyme | 237 |
Chapter 13 Autonomic and Sensory Neuron Cultures | 249 |
Chapter 14 Retinal Cultures | 265 |
Chapter 15 Migration and Adhesion Assays | 285 |
Techniques for Study of Early Neurogenesis and Myogenesis | 301 |
Chapter 17 TimeLapse Cinephotomicrogaphy Videogaphy and Videomicrography of the Avian Blastoderm ... | 331 |
Index | 355 |
Volumes in Series | 365 |
Chapter 10 Manipulating Gene Expression with ReplicationCompetent Retroviruses | 185 |
Chapter 11 In Situ Hybridization Analysis of Chick Embryos in Whole Mount and Tissue Sections ... | 219 |
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Methods in Avian Embryology Marianne Bronner-Fraser,Paul T. Matsudaira Anteprima non disponibile - 1996 |
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Brani popolari
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