Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis

Copertina anteriore
W. T. Mason, William T. Mason
Elsevier Science, 10 mag 1999 - 647 pagine
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.
The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.

TOPICS COVERED:
* Single and multiphoton confocal microscopy
* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies
* Applications of new optical probes for imaging proteins in gels
* Probes and detection technologies for imaging membrane potential in live cells
* Use of optical probes to detect microorganisms
* Raman and confocal raman microspectroscopy
* Fluorescence lifetime imaging microscopy
* Digital CCD cameras and their application in biological microscopy

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Informazioni sull'autore (1999)

Dr. Bill Mason founded Life Science Resources in 1992 and until recently was Head of the Laboratory of Neural and Secretory Signalling at the Babraham Institute in Cambridge. He is recognised internationally as a leader in innovative R&D in real time, low light level imaging. Dr. Mason was awarded his PhD from the Physiological Laboratory in the University of Cambridge. He is also a Member of Trinity College, Cambridge. In addition to his academic and commercial interests, Dr. Mason acts as an expert scientific adviser for strategic and funding advisory bodies in the biomedical sciences including Medical Research Council (UK), NSF (USA), the Welcome Trust, the Nuffield Foundation and the Commission of European Communities. He has played a major role in UK science and technology strategy as a Member of the Working Group on Higher Education and Highly Qualified Manpower for the Advisory Council on Science and Technology (ACOST) at the UK Cabinet Office in H.M. Government, where he co-authored consultative documents relating to government policy on education and training in science and technology.

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