A Manual of Microbiological Methods: By Michael P. Mauzy and Others

15 minutes 6-ounce water sample Agar agar medium algae ammonium Autoclave Autoclave at 15 bacteriological Benthic bottle brilliant green lactose broth buffered distilled water chlorine Citrate coliform group colonies color concentration containing counting cell culture Dipotassium hydrogen phosphate Dissolve distilled water Eosin Methylene Blue Examination of Water fecal coliform fermentation tubes Final pH flasks g 1 liter g Sodium Glucose Gram stain Gram-negative green lactose bile growth hours at 35 Incubate inhibitory inoculate inoculum K₂HPO4 laboratory lactose bile broth lauryl tryptose broth liter Dispense MATERIALS membrane filter microorganisms microscope minutes at 15 ml of sample negative nematodes Non-fecal number of organisms nutrient Peptone petri dishes plankton pollution Potassium Potassium dihydrogen phosphate probable number PROCEDURE reagent REPORTING Safranin sewage slide Sodium chloride sodium thiosulfate solution spores standard 6-ounce water Standard Methods substance Sulfate Reducing Sulfate Reducing Anaerobes sulfur temperature Thiobacillus volume Wastewater 13th Edition Water and Wastewater Yeast extract