Cell and Tissue Culture

acid agar amino-acids amnion animal cells antibiotics apparatus ascites aseptic autoclave balanced salt solution Biochem Biol blood Cancer Inst Cancer Res Carrel cell cultures Cell Res cell strains cell suspension cells in tissue cells in vitro cent centrifuge chamber chick embryo chick heart chromosomes CO₂ concentration containing coverslip cultivation cultured cells developed dilute effect embryo embryo extract essential explants fibroblasts filters flask fluid glass glassware glucose glutamine grow grown growth HeLa human incubator inoculated laboratory large number mammalian cells material medium membrane metabolism method mitosis mouse N.Y. Acad Nature Lond normal obtained organ culture osmotic pressure Pasteur pipette Petri dish Physiol pipette poliomyelitis PPLO prepared Proc protein removed rinsed serum slide sodium sterile sterilised stock solution studies survival synthetic technique temperature test-tubes Thymine tion tissue culture transplantation trypsin trypsinisation tube tumour usually virus viruses vitamin vitro washed