Molecular Cloning: A Laboratory Manual, Volume 1Cold Spring Harbor Laboratory, 1989 - 676 pagine |
Dall'interno del libro
Risultati 1-3 di 33
Pagina 2-28
... Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI 45770 Rend 39160 Smal 38880 Bgill 38820 Bghl 38170 Bg / l 37940 Pvul 37860 Bgl 34830 San 34590 San 36140 Sall 35150 EcoRI 35140 Sacl Smal 35130 Xbal 35120 Sall 34070 Sall 33100 Sall 35100 HindIII ...
... Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI 45770 Rend 39160 Smal 38880 Bgill 38820 Bghl 38170 Bg / l 37940 Pvul 37860 Bgl 34830 San 34590 San 36140 Sall 35150 EcoRI 35140 Sacl Smal 35130 Xbal 35120 Sall 34070 Sall 33100 Sall 35100 HindIII ...
Pagina 2-38
... Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI b189 ninL44 D KH54 rex cl nin5 41600 Rend 35790 Smal 35020 Bg / l 34960 Bgill 34310 Bgl 34080 Pvul 34000 Bgill 32790 Xbal 32790 Sacl 32770 Sall 32760 Xhol 32760 EcoRI 32760 Xbal 29160 Bgl 29020 ...
... Kpnl 17050 Kpnl 11930 Pvul 5510 BamHI b189 ninL44 D KH54 rex cl nin5 41600 Rend 35790 Smal 35020 Bg / l 34960 Bgill 34310 Bgl 34080 Pvul 34000 Bgill 32790 Xbal 32790 Sacl 32770 Sall 32760 Xhol 32760 EcoRI 32760 Xbal 29160 Bgl 29020 ...
Pagina 2-50
... Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI | M | L | K |||| H ICLO E JAUZUVGT B 101 exo ber 37030 Smal 35960 Bg / ll 35900 Bg / ll 33110 Xhol 32750 Sall 32260 Sall 31230 Smal 26670 Pvul 24730 Smal 24580 Pvul 23850 Pvul 23370 Pvul 22920 Pvul ...
... Kpnl 17050 Kpnl 11930 Pvul 6510 BamHI | M | L | K |||| H ICLO E JAUZUVGT B 101 exo ber 37030 Smal 35960 Bg / ll 35900 Bg / ll 33110 Xhol 32750 Sall 32260 Sall 31230 Smal 26670 Pvul 24730 Smal 24580 Pvul 23850 Pvul 23370 Pvul 22920 Pvul ...
Sommario
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Parole e frasi comuni
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml