Molecular Cloning: A Laboratory Manual, Volume 1Cold Spring Harbor Laboratory, 1989 - 676 pagine |
Dall'interno del libro
Risultati 1-3 di 52
Pagina 1-40
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
Pagina 4-29
... supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual supernatant . A tiny pellet of precipitated bac- teriophage particles should be visible at the ...
... supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual supernatant . A tiny pellet of precipitated bac- teriophage particles should be visible at the ...
Pagina 4-32
... supernatant to drain away from the precipitate . Use Kimwipes to remove the last traces of supernatant from the walls and neck of the bottle . 4. Add 10 ml of a solution of 10 mm Tris Cl ( pH 8.0 ) to the bottle . Use a pasteur pipette ...
... supernatant to drain away from the precipitate . Use Kimwipes to remove the last traces of supernatant from the walls and neck of the bottle . 4. Add 10 ml of a solution of 10 mm Tris Cl ( pH 8.0 ) to the bottle . Use a pasteur pipette ...
Sommario
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Altre edizioni - Visualizza tutto
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,E. F. Fritsch,Tom Maniatis Visualizzazione estratti - 1989 |
Parole e frasi comuni
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml