Molecular Cloning: A Laboratory Manual, Libro 2Cold Spring Harbor Laboratory, 1989 - 654 pagine |
Dall'interno del libro
Risultati 1-3 di 5
Pagina 9-7
... foreign DNA . This increased capacity allows a modest reduction in the number of recombinant clones that need be constructed and screened to isolate a particular segment of the eukaryotic genome . More significant , however , are the ...
... foreign DNA . This increased capacity allows a modest reduction in the number of recombinant clones that need be constructed and screened to isolate a particular segment of the eukaryotic genome . More significant , however , are the ...
Pagina 9-8
... segments of foreign DNA . In some cases , the sequence of the stuffer fragment may not be available , but this is usually not relevant . • The presence of amber mutations in the arms . Amber mutations are carried in essential genes in ...
... segments of foreign DNA . In some cases , the sequence of the stuffer fragment may not be available , but this is usually not relevant . • The presence of amber mutations in the arms . Amber mutations are carried in essential genes in ...
Pagina 15-77
... segment of foreign DNA after mutagenesis to ensure that no deletions or other types of mutations have occurred at sites other than the immediate target sequence . 6. Measure the volume of the bacteriophage suspension , and then add 0.25 ...
... segment of foreign DNA after mutagenesis to ensure that no deletions or other types of mutations have occurred at sites other than the immediate target sequence . 6. Measure the volume of the bacteriophage suspension , and then add 0.25 ...
Altre edizioni - Visualizza tutto
Molecular Cloning: A Laboratory Manual, Libro 2 Joseph Sambrook,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Libro 2 Joseph Sambrook,Tom Maniatis Visualizzazione estratti - 1989 |
Molecular Cloning: A Laboratory Manual, Libro 2 Joseph Sambrook,Tom Maniatis Visualizzazione estratti - 1989 |
Parole e frasi comuni
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector