Animal Tissue Culture: Advances in TechniqueGerald D. Wasley Butterworths, 1972 - 192 pagine |
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Pagina 50
... dilution ( equal parts of dilutant and diluent ) is most commonly employed for serum assays , and the tenfold dilution is often ( but not exclusively ) used for virus titration . Whereas conventional dilutions utilise the glass pipette ...
... dilution ( equal parts of dilutant and diluent ) is most commonly employed for serum assays , and the tenfold dilution is often ( but not exclusively ) used for virus titration . Whereas conventional dilutions utilise the glass pipette ...
Pagina 55
... DILUTION CYCLE To enable the operator to maintain a uniform method for manipulating the diluters , a sequence of steps ( dilution cycle ) was described by Sever ' . This cycle has been modified by our laboratory and is shown in Fig ...
... DILUTION CYCLE To enable the operator to maintain a uniform method for manipulating the diluters , a sequence of steps ( dilution cycle ) was described by Sever ' . This cycle has been modified by our laboratory and is shown in Fig ...
Pagina 63
... dilution increments , however , greater volumes can be sub- stituted provided the total dilution volume contains sufficient nutrient for cell growth . The virus or serum assay technique is diagrammed in Fig . 3.11a and a typical ...
... dilution increments , however , greater volumes can be sub- stituted provided the total dilution volume contains sufficient nutrient for cell growth . The virus or serum assay technique is diagrammed in Fig . 3.11a and a typical ...
Sommario
Mammalian Cell Culture Media | 1 |
Sterile Filtration | 21 |
Techniques for Cell Cultivation in Plastic Microtitration Plates and | 49 |
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alcohol amino acids amniotic fluid ampoules animal antibiotics antibody antigen asbestos asbestos pad assay autoclaving batches Biol bottles bubble point buffer Carrel flask cell cultures cell lines cell monolayers cell suspension centrifuge chamber chromosome concentration conjugate containing cover slips cover-glass culture media culture vessel cytoplasm depth filter diameter diluent dilution Expl Cell Res fibroblasts filter holder filtration system fluorescent foetal freezing glass growth medium human incubation infection inoculated inverted isolation laboratory latter liquid nitrogen litres macrophages membrane filter metaphase method microplate microscope Millipore monkey kidney cells monolayer myxovirus needle neutralisation test normal obtained particles Petri dish plasma plastic plate pore prefilter preparation pressure Proc procedure produce protein Reagents removed rinsed Rosenbaum rubber bung serum specimens staining sterile sterilising sterilising filtration storage subculture suitable surface technique temperature tissue culture toxic trypsin tubes viral virus viruses Vitamin volume washed