Animal Tissue Culture: Advances in TechniqueGerald D. Wasley Butterworths, 1972 - 192 pagine |
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Pagina 64
... or metabolic - inhibition . Procedure Mix reagents ; cover plate ( s ) with lid ; incubate ( 30-120 min ) suspension Incubation 0-025 As above * MTCD50 = U ml Virus Cells 2 .250 Fig . 3.11 . 64 TECHNIQUES FOR CELL CULTIVATION.
... or metabolic - inhibition . Procedure Mix reagents ; cover plate ( s ) with lid ; incubate ( 30-120 min ) suspension Incubation 0-025 As above * MTCD50 = U ml Virus Cells 2 .250 Fig . 3.11 . 64 TECHNIQUES FOR CELL CULTIVATION.
Pagina 117
... incubation to be started . If a conventional erect microscope is to be used for the observation of the cells the incubation must begin with the chamber inverted so that the cells shall deposit on the upper wall . After 2-3 hours in the ...
... incubation to be started . If a conventional erect microscope is to be used for the observation of the cells the incubation must begin with the chamber inverted so that the cells shall deposit on the upper wall . After 2-3 hours in the ...
Pagina 155
... incubation at 37 ° C for about 30 min , with occasional agitation of the fluid by repeatedly sucking it into a ... incubating 3-7 days before embedding as described below . By using this preliminary incubating stage before embed- ding ...
... incubation at 37 ° C for about 30 min , with occasional agitation of the fluid by repeatedly sucking it into a ... incubating 3-7 days before embedding as described below . By using this preliminary incubating stage before embed- ding ...
Sommario
Mammalian Cell Culture Media | 1 |
Sterile Filtration | 21 |
Techniques for Cell Cultivation in Plastic Microtitration Plates and | 49 |
Copyright | |
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alcohol amino acids amniotic fluid ampoules animal antibiotics antibody antigen asbestos asbestos pad assay autoclaving batches Biol bottles bubble point buffer Carrel flask cell cultures cell lines cell monolayers cell suspension centrifuge chamber chromosome concentration conjugate containing cover slips cover-glass culture media culture vessel cytoplasm depth filter diameter diluent dilution Expl Cell Res fibroblasts filter holder filtration system fluorescent foetal freezing glass growth medium human incubation infection inoculated inverted isolation laboratory latter liquid nitrogen litres macrophages membrane filter metaphase method microplate microscope Millipore monkey kidney cells monolayer myxovirus needle neutralisation test normal obtained particles Petri dish plasma plastic plate pore prefilter preparation pressure Proc procedure produce protein Reagents removed rinsed Rosenbaum rubber bung serum specimens staining sterile sterilising sterilising filtration storage subculture suitable surface technique temperature tissue culture toxic trypsin tubes viral virus viruses Vitamin volume washed