Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time AnalysisW. T. Mason Elsevier, 16 apr 1999 - 647 pagine The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy |
Sommario
| 15 | |
| 63 | |
| 115 | |
Technology for Qualitative and Quantitative Detection of Optical Probes in Living Cells | 173 |
Using Novel Indications for Genetic Molecular and Cellular Function | 249 |
Applications of Confocal Microscopy for Optical Probe Imaging | 301 |
Advanced Imaging and Light Detection Approaches for Optical Probe Applications | 375 |
CCD Cameras Key Enabling Technologies for Optical Probe Imaging | 489 |
Flow Cytometric Methodologies for Measurement of Optical Probes in Live Cells | 525 |
Applications of Optical Probe Imaging to Biological Problems | 567 |
Index | 634 |
Color Plate Section | 649 |
Altre edizioni - Visualizza tutto
Fluorescent and Luminescent Probes for Biological Activity: A Practical ... W. T. Mason Visualizzazione estratti - 1993 |
Fluorescent and Luminescent Probes for Biological Activity: A Practical ... W. T. Mason Anteprima non disponibile - 1993 |
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