Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time AnalysisW. T. Mason Elsevier, 16 apr 1999 - 647 pagine The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy |
Dall'interno del libro
Risultati 1-5 di 85
Pagina ii
... Methods in Modern Biology Series Editor DAVID B SATTELLE Computer Analysis of Electrophysiological Signals J ... Methods for Intracellular Recording and Ionophoresis RD Purves Immunochemical Methods in Cell and Molecular Biology RJ Mayer ...
... Methods in Modern Biology Series Editor DAVID B SATTELLE Computer Analysis of Electrophysiological Signals J ... Methods for Intracellular Recording and Ionophoresis RD Purves Immunochemical Methods in Cell and Molecular Biology RJ Mayer ...
Pagina v
... method starts to become more widely appreciated, and therefore adopted by scientists with a diversity of backgrounds ... methods sections of journal papers. The books will be of value to advanced researchers and graduate students seeking ...
... method starts to become more widely appreciated, and therefore adopted by scientists with a diversity of backgrounds ... methods sections of journal papers. The books will be of value to advanced researchers and graduate students seeking ...
Pagina 19
... methods (Kasten, 1983b). Other dyes produced during this period included xanthene and acridine derivatives, which were highly ... method for * In 1897, the Grübler firm became known as Dr K. Hollborn & Söhne. Table 2.1 Common biological ...
... methods (Kasten, 1983b). Other dyes produced during this period included xanthene and acridine derivatives, which were highly ... method for * In 1897, the Grübler firm became known as Dr K. Hollborn & Söhne. Table 2.1 Common biological ...
Pagina 22
... methods. At this time, the word fluorochrome was created to mean fluorescent compounds which are bound selectively to individual tissue structures without disturbing the autofluorescence of other tissue elements (Haitinger, 1934). To ...
... methods. At this time, the word fluorochrome was created to mean fluorescent compounds which are bound selectively to individual tissue structures without disturbing the autofluorescence of other tissue elements (Haitinger, 1934). To ...
Pagina 23
... method than the ZiehlNeelsen procedure to detect tubercle bacilli. Eventually, auramine O (CI 41000) was found satisfactory in combination with an acid-alcohol treatment of the smears (Hagemann, 1938). Auramine O produced brilliant ...
... method than the ZiehlNeelsen procedure to detect tubercle bacilli. Eventually, auramine O (CI 41000) was found satisfactory in combination with an acid-alcohol treatment of the smears (Hagemann, 1938). Auramine O produced brilliant ...
Sommario
15 | |
63 | |
115 | |
Technology for Qualitative and Quantitative Detection of Optical Probes in Living Cells | 173 |
Using Novel Indications for Genetic Molecular and Cellular Function | 249 |
Applications of Confocal Microscopy for Optical Probe Imaging | 301 |
Advanced Imaging and Light Detection Approaches for Optical Probe Applications | 375 |
CCD Cameras Key Enabling Technologies for Optical Probe Imaging | 489 |
Flow Cytometric Methodologies for Measurement of Optical Probes in Live Cells | 525 |
Applications of Optical Probe Imaging to Biological Problems | 567 |
Index | 634 |
Color Plate Section | 649 |
Altre edizioni - Visualizza tutto
Fluorescent and Luminescent Probes for Biological Activity: A Practical ... W. T. Mason Visualizzazione estratti - 1993 |
Fluorescent and Luminescent Probes for Biological Activity: A Practical ... W. T. Mason Anteprima non disponibile - 1993 |
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