Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
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Dall'interno del libro
Risultati 1-5 di 58
Pagina 32
... buffer (=TENS buffer): 100 mM Tris-HCI (pH 8.0) 100 mM NaCl l rnM EDTA 0.5% Sodium dodecyl sulfate (SDS) B. LABELING 32 Karl-Erik lohansson.
... buffer (=TENS buffer): 100 mM Tris-HCI (pH 8.0) 100 mM NaCl l rnM EDTA 0.5% Sodium dodecyl sulfate (SDS) B. LABELING 32 Karl-Erik lohansson.
Pagina 38
... buffer (10x) 2.0 ul DNA (about 200 ng) . . . . p.I T4 PNK (10 U/p.l) 0.7 ul ['1'-32P]ATP (1.85 MBq, >185 MBq/mmol) 5.0 p.l Final volume 20.0 pl The amount of water is dependent on the concentration of. 1. Add the following components to ...
... buffer (10x) 2.0 ul DNA (about 200 ng) . . . . p.I T4 PNK (10 U/p.l) 0.7 ul ['1'-32P]ATP (1.85 MBq, >185 MBq/mmol) 5.0 p.l Final volume 20.0 pl The amount of water is dependent on the concentration of. 1. Add the following components to ...
Pagina 39
... buffer. 2. Remove the excess of radioactive ATP by gel filtration on a NAP-5 column equilibrated with elution buffer. Work behind the plexiscreen. Apply the sample onto the gel bed and elute it into the column with 400 pl of elution buffer ...
... buffer. 2. Remove the excess of radioactive ATP by gel filtration on a NAP-5 column equilibrated with elution buffer. Work behind the plexiscreen. Apply the sample onto the gel bed and elute it into the column with 400 pl of elution buffer ...
Pagina 61
... buffer: 1 mM EDTA, pH 8.0; 10 mM Tris—HCl, pH 8.0; 0.1% Triton X-100; 200 1.tg/ ml proteinase K 61 Molecular and Diagnostic Procedures in Mycoplasmology, Vol. 11 Copyright © 1996 by Academic Press, Inc. All rights of reproduction in any ...
... buffer: 1 mM EDTA, pH 8.0; 10 mM Tris—HCl, pH 8.0; 0.1% Triton X-100; 200 1.tg/ ml proteinase K 61 Molecular and Diagnostic Procedures in Mycoplasmology, Vol. 11 Copyright © 1996 by Academic Press, Inc. All rights of reproduction in any ...
Pagina 62
... buffer (pH 7.2) containing 5% fetal calf serum] or in mycoplasmal culture medium. 2. Cellular material from fluids, such as bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF), is recovered by centrifugation (3500 g for 15 ...
... buffer (pH 7.2) containing 5% fetal calf serum] or in mycoplasmal culture medium. 2. Cellular material from fluids, such as bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF), is recovered by centrifugation (3500 g for 15 ...
Sommario
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
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Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
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1996 by Academic Academic Press Acholeplasma agar amplification animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma diagnosis Diagnostic Procedures dilution disease DNA probes ELISA experimental fermentans filter fluid fluorescence form reserved gallisepticum gene growth hominis host human hybridization hyopneumoniae hyorhinis identification immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory leafhoppers medium membrane Methods in Mycoplasmology mice Microbiol microscope Molecular and Diagnostic mollicutes myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae Mycoplasma pulmonis mycoplasma species nitrocellulose oligonucleotide organisms pathogenic phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein pulmonis rRNA samples sensitivity sequence serological serum solution specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb