Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
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Dall'interno del libro
Risultati 1-5 di 37
Pagina 27
... primers of wide specificity (“universal primers”) which will react with the DNA of any mycoplasma or even with the DNA of other prokaryotes. This may be satisfactory for detection of mycoplasma infection in cell cultures, where the goal ...
... primers of wide specificity (“universal primers”) which will react with the DNA of any mycoplasma or even with the DNA of other prokaryotes. This may be satisfactory for detection of mycoplasma infection in cell cultures, where the goal ...
Pagina 31
... primers or as targets for polymerase chain reaction (PCR) primers. Biotinylated PCR products can be produced for automated solidphase sequencing by which sequence data of rRNA genes can be generated very efficiently (Pettersson et al ...
... primers or as targets for polymerase chain reaction (PCR) primers. Biotinylated PCR products can be produced for automated solidphase sequencing by which sequence data of rRNA genes can be generated very efficiently (Pettersson et al ...
Pagina 34
... primers complementary to universal regions (see Chapter E3, Vol. I). It is sometimes sufficient to make a partial. Prepare the herring (salmon) sperm DNA solution as described by Sambrook et al. (1989). 1. Add the following components to ...
... primers complementary to universal regions (see Chapter E3, Vol. I). It is sometimes sufficient to make a partial. Prepare the herring (salmon) sperm DNA solution as described by Sambrook et al. (1989). 1. Add the following components to ...
Pagina 41
... primers complementary to the V2 and the V8 regions of the 16S rRNA gene of Mycoplasma hyopneumoniae (Mattsson et al., 1995). The filter was hybridized with the Mhp6/30 probe (see Tables I and II) labeled with (A) 32P and (B) digoxigenin ...
... primers complementary to the V2 and the V8 regions of the 16S rRNA gene of Mycoplasma hyopneumoniae (Mattsson et al., 1995). The filter was hybridized with the Mhp6/30 probe (see Tables I and II) labeled with (A) 32P and (B) digoxigenin ...
Pagina 51
... primers for its amplification (Harasawa et al., 1991; Brogan et al., 1992). References. Abiven, P., Blanchard, B., Saillard, C., Kobisch, M., and Bové, I. M. (1992). A specific DNA probe for detecting Mycoplasma hyopneumaniae in ...
... primers for its amplification (Harasawa et al., 1991; Brogan et al., 1992). References. Abiven, P., Blanchard, B., Saillard, C., Kobisch, M., and Bové, I. M. (1992). A specific DNA probe for detecting Mycoplasma hyopneumaniae in ...
Sommario
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
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Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
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1996 by Academic Academic Press Acholeplasma agar amplification animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma diagnosis Diagnostic Procedures dilution disease DNA probes ELISA experimental fermentans filter fluid fluorescence form reserved gallisepticum gene growth hominis host human hybridization hyopneumoniae hyorhinis identification immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory leafhoppers medium membrane Methods in Mycoplasmology mice Microbiol microscope Molecular and Diagnostic mollicutes myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae Mycoplasma pulmonis mycoplasma species nitrocellulose oligonucleotide organisms pathogenic phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein pulmonis rRNA samples sensitivity sequence serological serum solution specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb