Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
|
Dall'interno del libro
Risultati 1-5 di 32
Pagina v
... rRNA Karl-Erik /ohansson Cloned Genomic DNA Fragments as Probes David Yogev and Shmuel Razin PCR: Selection of Target Sequences Rémi Kovacic, Odile Crau, and Alain Blanchard PCR: Preparation of DNA from Clinical Specimens Bertille de ...
... rRNA Karl-Erik /ohansson Cloned Genomic DNA Fragments as Probes David Yogev and Shmuel Razin PCR: Selection of Target Sequences Rémi Kovacic, Odile Crau, and Alain Blanchard PCR: Preparation of DNA from Clinical Specimens Bertille de ...
Pagina 25
... rRNA genes were effective in detecting and identifying mycoplasmas infecting cell cultures by Southern blot hybridization with labeled pMC5 as a probe (Razin et al., 1984). In this way, 1 ng of mycoplasmal DNA, equivalent to about 105 ...
... rRNA genes were effective in detecting and identifying mycoplasmas infecting cell cultures by Southern blot hybridization with labeled pMC5 as a probe (Razin et al., 1984). In this way, 1 ng of mycoplasmal DNA, equivalent to about 105 ...
Pagina 26
... rRNA genes can be applied. When labeled, they can be used as probes in dot-blot hybridization with rRNA of the clinical specimens suspected of carrying the mycoplasma for which the probe was developed (Chapter A2, this volume). Since ...
... rRNA genes can be applied. When labeled, they can be used as probes in dot-blot hybridization with rRNA of the clinical specimens suspected of carrying the mycoplasma for which the probe was developed (Chapter A2, this volume). Since ...
Pagina 27
... rRNA genes carry, in addition to the conserved regions, more specific variable regions, as well as specific 16S-23S intergenic spacer regions. Primers can be selected from these regions with various degrees of specificity, ranging from ...
... rRNA genes carry, in addition to the conserved regions, more specific variable regions, as well as specific 16S-23S intergenic spacer regions. Primers can be selected from these regions with various degrees of specificity, ranging from ...
Pagina 28
... rRNA for rapid detection of mycoplasma contamination in cell cultures. FEMS Microbiol. Lett. 107, 139-144. Persing, D. H., Smith, T. F., Tenover, F. C., and White, T. J. (1993). “Diagnostic Molecular Microbiology: Principles and ...
... rRNA for rapid detection of mycoplasma contamination in cell cultures. FEMS Microbiol. Lett. 107, 139-144. Persing, D. H., Smith, T. F., Tenover, F. C., and White, T. J. (1993). “Diagnostic Molecular Microbiology: Principles and ...
Sommario
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
Altre edizioni - Visualizza tutto
Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
Parole e frasi comuni
1996 by Academic Academic Press Acholeplasma agar amplification animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma diagnosis Diagnostic Procedures dilution disease DNA probes ELISA experimental fermentans filter fluid fluorescence form reserved gallisepticum gene growth hominis host human hybridization hyopneumoniae hyorhinis identification immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory leafhoppers medium membrane Methods in Mycoplasmology mice Microbiol microscope Molecular and Diagnostic mollicutes myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae Mycoplasma pulmonis mycoplasma species nitrocellulose oligonucleotide organisms pathogenic phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein pulmonis rRNA samples sensitivity sequence serological serum solution specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb