Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
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Dall'interno del libro
Risultati 1-5 di 82
Pagina 26
... samples, when small numbers of mycoplasmas are often present. Another class of DNA probes is that consisting of chromosomal segments specific for a certain mollicute species. These segments are derived from a genomic library of the ...
... samples, when small numbers of mycoplasmas are often present. Another class of DNA probes is that consisting of chromosomal segments specific for a certain mollicute species. These segments are derived from a genomic library of the ...
Pagina 27
... sample, following its concentration by centrifugation, is often sufficient to make the organisms' DNA accessible. However, some clinical samples may contain undefined inhibitors of the PCR reaction, reducing the efficiency of ...
... sample, following its concentration by centrifugation, is often sufficient to make the organisms' DNA accessible. However, some clinical samples may contain undefined inhibitors of the PCR reaction, reducing the efficiency of ...
Pagina 33
... samples) Hybridization oven equipped with a rotisserie Glass vials to be used in the hybridization oven Thermostatted waterbath X-ray films (X-OMAT AR film from Eastman Kodak Co., Rochester, NY) X-ray film cassettes with intensifying ...
... samples) Hybridization oven equipped with a rotisserie Glass vials to be used in the hybridization oven Thermostatted waterbath X-ray films (X-OMAT AR film from Eastman Kodak Co., Rochester, NY) X-ray film cassettes with intensifying ...
Pagina 39
... sample onto the gel bed and elute it into the column with 400 pl of elution buffer. Collect the first 500 pl which ... samples can be conveniently diluted in an ELISA microtiter plate with an 8- or 12-channel pipette. Be careful to ...
... sample onto the gel bed and elute it into the column with 400 pl of elution buffer. Collect the first 500 pl which ... samples can be conveniently diluted in an ELISA microtiter plate with an 8- or 12-channel pipette. Be careful to ...
Pagina 40
... samples consisting of pure nucleic acid preparations or PCR products (Fig. 2B). The PCR products have to be transformed to single-stranded DNA by heating the sample in a total volume of 0.5 ml of 0.4 M NaOH and 10 mM EDTA at 100°C for ...
... samples consisting of pure nucleic acid preparations or PCR products (Fig. 2B). The PCR products have to be transformed to single-stranded DNA by heating the sample in a total volume of 0.5 ml of 0.4 M NaOH and 10 mM EDTA at 100°C for ...
Sommario
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
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Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
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1996 by Academic Academic Press Acholeplasma agar amplification animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma diagnosis Diagnostic Procedures dilution disease DNA probes ELISA experimental fermentans filter fluid fluorescence form reserved gallisepticum gene growth hominis host human hybridization hyopneumoniae hyorhinis identification immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory leafhoppers medium membrane Methods in Mycoplasmology mice Microbiol microscope Molecular and Diagnostic mollicutes myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae Mycoplasma pulmonis mycoplasma species nitrocellulose oligonucleotide organisms pathogenic phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein pulmonis rRNA samples sensitivity sequence serological serum solution specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb