Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
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Dall'interno del libro
Risultati 1-5 di 83
Pagina 16
... serum-free medium Positive Genus Acholeplasma Negative Other mollicute D. Growth in serum-free medium containing 0.04% Tween 80 Positive Genus Mesoplasma Negative Other mollicute E. Optimal growth temperature 30°—32°C Genus Entomoplasma ...
... serum-free medium Positive Genus Acholeplasma Negative Other mollicute D. Growth in serum-free medium containing 0.04% Tween 80 Positive Genus Mesoplasma Negative Other mollicute E. Optimal growth temperature 30°—32°C Genus Entomoplasma ...
Pagina 17
... serum-free medium formulations are given in Chapter E7 (Volume 1). Determination of optimum growth temperature differentiates Mycoplasma and Ureaplasma species of vertebrates, those growing best between 35° and 37°C, from Entomoplasma ...
... serum-free medium formulations are given in Chapter E7 (Volume 1). Determination of optimum growth temperature differentiates Mycoplasma and Ureaplasma species of vertebrates, those growing best between 35° and 37°C, from Entomoplasma ...
Pagina 34
... serum albumin (Fraction V from Sigma Chemical Co.) Dissolve the components in autoclaved water to a final volume of 500 ml, filter the solution, dispense into aliquots, and store at —20°C. SSC (20><): 3.0M NaCl (175.3 g) 0.3 M sodium ...
... serum albumin (Fraction V from Sigma Chemical Co.) Dissolve the components in autoclaved water to a final volume of 500 ml, filter the solution, dispense into aliquots, and store at —20°C. SSC (20><): 3.0M NaCl (175.3 g) 0.3 M sodium ...
Pagina 62
... serum] or in mycoplasmal culture medium. 2. Cellular material from fluids, such as bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF), is recovered by centrifugation (3500 g for 15 minutes). The deposit is resuspended in 1 ml of ...
... serum] or in mycoplasmal culture medium. 2. Cellular material from fluids, such as bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF), is recovered by centrifugation (3500 g for 15 minutes). The deposit is resuspended in 1 ml of ...
Pagina 67
... serum albumin and nonionic detergents such as Tween 20 or Triton X-100 are included to stabilize the enzyme. Generally, the assay buffer is supplied with the Taq DNA polymerase by the manufacturer. CYCLE CONDITIONS, TIME, AND ...
... serum albumin and nonionic detergents such as Tween 20 or Triton X-100 are included to stabilize the enzyme. Generally, the assay buffer is supplied with the Taq DNA polymerase by the manufacturer. CYCLE CONDITIONS, TIME, AND ...
Sommario
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
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Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
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1996 by Academic Academic Press Acholeplasma agar amplification animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma diagnosis Diagnostic Procedures dilution disease DNA probes ELISA experimental fermentans filter fluid fluorescence form reserved gallisepticum gene growth hominis host human hybridization hyopneumoniae hyorhinis identification immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory leafhoppers medium membrane Methods in Mycoplasmology mice Microbiol microscope Molecular and Diagnostic mollicutes myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae Mycoplasma pulmonis mycoplasma species nitrocellulose oligonucleotide organisms pathogenic phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein pulmonis rRNA samples sensitivity sequence serological serum solution specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb