Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic ProceduresAcademic Press, 21 feb 1996 - 466 pagine This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.
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Pagina 75
... Sterile water, injection or irrigation grade (Note: Conventional distilled water may occasionally be contaminated with trace amounts of prokaryotic DNA) 75 Molecular and Diagnostic Procedures in Mycoplasmology. Vol. 11 Copyright © 1996 ...
... Sterile water, injection or irrigation grade (Note: Conventional distilled water may occasionally be contaminated with trace amounts of prokaryotic DNA) 75 Molecular and Diagnostic Procedures in Mycoplasmology. Vol. 11 Copyright © 1996 ...
Pagina 76
... Sterile water, injection or irrigation grade dNTPs solution, 2.5 mM each Primers for the first-round PCR Primer F1 [5'-ACACCATGGGAG(C/T)TGGTAAT-3'], 10 pmol/p.l Primer R1 [5 '-CTTC(A/T)TCGACTT(C/T)CAGACCCAAGGCAT-3'], 10 pmol/p.l Primers ...
... Sterile water, injection or irrigation grade dNTPs solution, 2.5 mM each Primers for the first-round PCR Primer F1 [5'-ACACCATGGGAG(C/T)TGGTAAT-3'], 10 pmol/p.l Primer R1 [5 '-CTTC(A/T)TCGACTT(C/T)CAGACCCAAGGCAT-3'], 10 pmol/p.l Primers ...
Pagina 77
... sterile Eppendorf tube prior to subculturing. [Notez Cell cultures grown for 3—5 days after passage are suitable for direct testing. Cell debris from frozen cell cultures may inhibit PCR. A single dose of lyophilized viral vaccines or ...
... sterile Eppendorf tube prior to subculturing. [Notez Cell cultures grown for 3—5 days after passage are suitable for direct testing. Cell debris from frozen cell cultures may inhibit PCR. A single dose of lyophilized viral vaccines or ...
Pagina 78
... sterile distilled water. 14. Measure the absorbance of the DNA solution at 260 and 280 nm, if necessary. (Note: Use 1 pl of DNA solution for this purpose.) 15. Dilute DNA to a final concentration between 50 and 250 ng/ pl so that the ...
... sterile distilled water. 14. Measure the absorbance of the DNA solution at 260 and 280 nm, if necessary. (Note: Use 1 pl of DNA solution for this purpose.) 15. Dilute DNA to a final concentration between 50 and 250 ng/ pl so that the ...
Pagina 82
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Sommario
1 | |
23 | |
Immunological Tools | 87 |
Antibiotic Sensitivity Testing | 179 |
Diagnosis of Specific Diseases | 205 |
Experimental Infections | 323 |
Diagnosis of Mycoplasma Infections of Cell Cultures | 405 |
Appendix | 453 |
Index | 463 |
Color Plate Section | 467 |
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Molecular and Diagnostic Procedures in Mycoplasmology: Vols. 1-2 Shmuel Razin,Joseph G. Tully Anteprima non disponibile - 1995 |
Parole e frasi comuni
1996 by Academic Academic Press Acholeplasma agar agar plate animals antibiotic antibody antigen antiserum arthritidis assay Avian Mycoplasma bacterial bovine broth buffer cell cultures cell lines centrifugation Chapter Clin clinical colonies conjugate containing detection of Mycoplasma dilution disease DNA probes ELISA experimental filter fluid fluorescence gallisepticum gene growth hominis host human hybridization hyopneumoniae immunofluorescence incubation inoculation inoculum isolated J. G. Tully laboratory medium membrane Methods in Mycoplasmology mice Microbial Microbiol microscope minutes Molecular and Diagnostic mollicutes monoclonal antibodies myco Mycoplasma genitalium Mycoplasma hominis Mycoplasma hyopneumoniae mycoplasma infection Mycoplasma pneumoniae mycoplasma species nitrocellulose oligonucleotide organisms pathogenic patients phytoplasma plant plasma plates polymerase chain reaction primers Procedures in Mycoplasmology protein rats Razin rights of reproduction rRNA samples sensitivity sequence serological serum solution specific specimens spiroplasmas sterile strains swab Taylor-Robinson techniques tion tissue titer tube Ureaplasma urealyticum urogenital volume washed Whitcomb