In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 98
... MEM supplemented with 2 × amino acids and vitamins , 4 mM L - glutamine , 15 % fetal bovine serum , and 50 μg / ml gentamicin ( 2 ) . Following subculture at a density of 1 x 10 ' cells per well of a 24 - well multiplate , the DMEM ...
... MEM supplemented with 2 × amino acids and vitamins , 4 mM L - glutamine , 15 % fetal bovine serum , and 50 μg / ml gentamicin ( 2 ) . Following subculture at a density of 1 x 10 ' cells per well of a 24 - well multiplate , the DMEM ...
Pagina 128
... DMEM per wash . About 0.5 ml of the last DMEM rinse is left in the plate to keep it moist . The debris - containing super- natant is carefully aspirated away after centrifugation , and the cell pellets are re- suspended in 3 ml of DMEM ...
... DMEM per wash . About 0.5 ml of the last DMEM rinse is left in the plate to keep it moist . The debris - containing super- natant is carefully aspirated away after centrifugation , and the cell pellets are re- suspended in 3 ml of DMEM ...
Pagina 295
... DMEM ( 1 : 1 ) , containing 10 mM HEPES , 0.1 U / ml penicillin Collagenase , 65–80 mg in 225 ml Ham's F12 / DMEM Pronase ( for postperfusion dispersal ) , 20 mg in 50 ml ... MEM 26. Isolation and Culture of Hepatic Nonparenchymal Cells 295.
... DMEM ( 1 : 1 ) , containing 10 mM HEPES , 0.1 U / ml penicillin Collagenase , 65–80 mg in 225 ml Ham's F12 / DMEM Pronase ( for postperfusion dispersal ) , 20 mg in 50 ml ... MEM 26. Isolation and Culture of Hepatic Nonparenchymal Cells 295.
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml