In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 5
... pipette ( such as a reversed Pasteur pipette ) . Slices cut by vibratome will consist of hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other ...
... pipette ( such as a reversed Pasteur pipette ) . Slices cut by vibratome will consist of hippocampus embedded in other tissue . These slices can be used as is , or the hippocampal portion can be dissected free . This and other ...
Pagina 51
... Pasteur pipettes and use a flame to reduce the bore size of one pipette to one - third of normal . Pour out an appropriate amount of plating medium into a flask and set aside ( 2.75 hemispheres / plate or 2.75 hemispheres / 9.6 ml of PM ...
... Pasteur pipettes and use a flame to reduce the bore size of one pipette to one - third of normal . Pour out an appropriate amount of plating medium into a flask and set aside ( 2.75 hemispheres / plate or 2.75 hemispheres / 9.6 ml of PM ...
Pagina 344
... Pasteur pipette and resus- pended in 35 ml DME - F12 . The tubules are washed three times with DME - F12 by centrifugation at 50 g for 2 min . The final pellet is resuspended in 150 ml DME - F12 , and samples are taken for protein ...
... Pasteur pipette and resus- pended in 35 ml DME - F12 . The tubules are washed three times with DME - F12 by centrifugation at 50 g for 2 min . The final pellet is resuspended in 150 ml DME - F12 , and samples are taken for protein ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml