In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 399
... Percoll ( % , v / v ) 551 45 " All buffers are equilibrated with 95 % O2 / 5 % CO2 by bubbling for 30 min after ... Percoll separation buffer ; C2 , Percoll wash buffer . Abbreviations : HEPES , 4- ( 2 - hydroxyethyl ) -1 ...
... Percoll ( % , v / v ) 551 45 " All buffers are equilibrated with 95 % O2 / 5 % CO2 by bubbling for 30 min after ... Percoll separation buffer ; C2 , Percoll wash buffer . Abbreviations : HEPES , 4- ( 2 - hydroxyethyl ) -1 ...
Pagina 460
... Percoll , first adjust the osmolality of Percoll with 1.5 M NaCl prepared in pyrogen - free distilled water , 1 part NaCl to 9 parts Percoll ( stock Percoll , ~ 340 mOsmol / kg water ) . Adjust the Percoll density to 50 % by mixing ...
... Percoll , first adjust the osmolality of Percoll with 1.5 M NaCl prepared in pyrogen - free distilled water , 1 part NaCl to 9 parts Percoll ( stock Percoll , ~ 340 mOsmol / kg water ) . Adjust the Percoll density to 50 % by mixing ...
Pagina 489
... Percoll gradient . Preparation of Percoll Gradients The gradients are prepared by adding 0.91 ml stock HBSS , 0.27 ml of 7.25 % NaCO3 , 0.14 ml HEPES , and 1.00 ml of 0.1 N HCl to four separate 16 - ml conical centrifuge tubes . Percoll ...
... Percoll gradient . Preparation of Percoll Gradients The gradients are prepared by adding 0.91 ml stock HBSS , 0.27 ml of 7.25 % NaCO3 , 0.14 ml HEPES , and 1.00 ml of 0.1 N HCl to four separate 16 - ml conical centrifuge tubes . Percoll ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml