In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 125
... reagents are required : 0.9 % NaCl ; 0.15 M KCI ; 0.1 M sodium phosphate buffer , pH 7.4 ; 0.2 M HEPES brought to pH 7.4 with 8 M NaOH ; glucose ; and gentamicin ( Whittaker M. A. Bioproducts , Walkersville , MD ) . All stock reagents ...
... reagents are required : 0.9 % NaCl ; 0.15 M KCI ; 0.1 M sodium phosphate buffer , pH 7.4 ; 0.2 M HEPES brought to pH 7.4 with 8 M NaOH ; glucose ; and gentamicin ( Whittaker M. A. Bioproducts , Walkersville , MD ) . All stock reagents ...
Pagina 376
... Reagents needed are methyl - a - D- [ U - 14 C ] glucopyranoside ( New England Nuclear , Boston , MA ) , unlabeled a - methylglucoside , and HBSS ( see above ) . y - Glutamyltransferase : Biochemical assays of y - glutamyltransferase in ...
... Reagents needed are methyl - a - D- [ U - 14 C ] glucopyranoside ( New England Nuclear , Boston , MA ) , unlabeled a - methylglucoside , and HBSS ( see above ) . y - Glutamyltransferase : Biochemical assays of y - glutamyltransferase in ...
Pagina 544
... reagents for , 295-296 purification of , 300-302 Hepatocytes . See also Human hepatocytes ; Liver cell cultures ; Rat hepatocytes from different laboratory species isolation and culture of , 255-261 advantages and disadvantages of , 260 ...
... reagents for , 295-296 purification of , 300-302 Hepatocytes . See also Human hepatocytes ; Liver cell cultures ; Rat hepatocytes from different laboratory species isolation and culture of , 255-261 advantages and disadvantages of , 260 ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml