In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 62
... aliquots and store at -20 ° C . Collagenase : Dissolve 50 mg collagenase ( type IIS , Sigma C - 1764 ) in 500 μl of Ringer's solution ; prepare 50 - μl aliquots and store at -20 ° C . DNase : Dissolve 7.6 mg of deoxyribonuclease ( type ...
... aliquots and store at -20 ° C . Collagenase : Dissolve 50 mg collagenase ( type IIS , Sigma C - 1764 ) in 500 μl of Ringer's solution ; prepare 50 - μl aliquots and store at -20 ° C . DNase : Dissolve 7.6 mg of deoxyribonuclease ( type ...
Pagina 63
... aliquots are stored at −20 ° C . Growth Media Basal L - 15 medium : An aliquot ( 50 ml ) of L - 15 supplement mix is thawed , dissolved in 500 ml of Leibowitz L - 15 medium ( GIBCO , 320-1415AJ ) , and stored at 4 ° C . Complete L - 15 ...
... aliquots are stored at −20 ° C . Growth Media Basal L - 15 medium : An aliquot ( 50 ml ) of L - 15 supplement mix is thawed , dissolved in 500 ml of Leibowitz L - 15 medium ( GIBCO , 320-1415AJ ) , and stored at 4 ° C . Complete L - 15 ...
Pagina 323
... aliquots for 0.1 % collagenase , and 0.5 - ml aliquots for 0.2 % DNase . Store at − 20 ° C . Do not refreeze after thawing . Procedure Oval Cell Isolation 1. Transfer liver to 100 - ml beaker containing 12.5 ml of 0.2 % collagenase ...
... aliquots for 0.1 % collagenase , and 0.5 - ml aliquots for 0.2 % DNase . Store at − 20 ° C . Do not refreeze after thawing . Procedure Oval Cell Isolation 1. Transfer liver to 100 - ml beaker containing 12.5 ml of 0.2 % collagenase ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml