In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 87
... assayed . At the time of assay , samples are quickly thawed and centrifuged ( 10,000 g ) in a microcentrifuge for 15 min , and the supernatants are placed in a 1 - ml micro- centrifuge tube for assay . To calibrate the assay , a ...
... assayed . At the time of assay , samples are quickly thawed and centrifuged ( 10,000 g ) in a microcentrifuge for 15 min , and the supernatants are placed in a 1 - ml micro- centrifuge tube for assay . To calibrate the assay , a ...
Pagina 476
... assay described above is applicable not only to murine splenocytes but also to cells obtained from tissues of other species , including human peripheral blood . It should be noted that human AB serum or combinations of human AB and FBS ...
... assay described above is applicable not only to murine splenocytes but also to cells obtained from tissues of other species , including human peripheral blood . It should be noted that human AB serum or combinations of human AB and FBS ...
Pagina 477
... assay termination , pulse each well with 1 μCi of [ 3H ] thymidine in a volume of 10 to 50 μl . 8. Harvest the ... assay apply for the MLR assay as well . In the case of a human MLR it is necessary to obtain a stimulator pool which ...
... assay termination , pulse each well with 1 μCi of [ 3H ] thymidine in a volume of 10 to 50 μl . 8. Harvest the ... assay apply for the MLR assay as well . In the case of a human MLR it is necessary to obtain a stimulator pool which ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml