In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 92
... Basal cytosolic calcium levels vary from 80 to 150 nM depending on growth conditions , time of harvest during the growth cycle , and the experimental con- ditions . Under normal conditions , basal cytosolic calcium levels should be ...
... Basal cytosolic calcium levels vary from 80 to 150 nM depending on growth conditions , time of harvest during the growth cycle , and the experimental con- ditions . Under normal conditions , basal cytosolic calcium levels should be ...
Pagina 105
... basal membrane has numerous infoldings that closely appose a basal lamina which forms an intimate part of Bruch's membrane . The basal membrane of cul- tured RPE makes spot attachments with the plastic surface of the culture plates ...
... basal membrane has numerous infoldings that closely appose a basal lamina which forms an intimate part of Bruch's membrane . The basal membrane of cul- tured RPE makes spot attachments with the plastic surface of the culture plates ...
Pagina 525
... basal cells surrounded by six or seven other basal cells . A single cell in this cen- tral subgroup may be the actively proliferating stem cell . Basal cells are of par- ticular interest as the target sites of tumor initiation and ...
... basal cells surrounded by six or seven other basal cells . A single cell in this cen- tral subgroup may be the actively proliferating stem cell . Basal cells are of par- ticular interest as the target sites of tumor initiation and ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml