In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 413
Charles A. Tyson, John M. Frazier. Cell Lines American Type Culture Collection ( Rockville , MD ) LLC - PK , ( CRL1392 ) LLC - RK , ( CCL106 ) Animals New Zealand White rabbits ( male , adult , Hazelton ... Cell Lines from Rabbit and Pig 413.
Charles A. Tyson, John M. Frazier. Cell Lines American Type Culture Collection ( Rockville , MD ) LLC - PK , ( CRL1392 ) LLC - RK , ( CCL106 ) Animals New Zealand White rabbits ( male , adult , Hazelton ... Cell Lines from Rabbit and Pig 413.
Pagina 417
... cell lines which have been extensively cultured or pas- saged for multiple generations , the degree of retention ( or loss ) of kidney- specific morphology and function is an important issue to consider ... Cell Lines from Rabbit and Pig 417.
... cell lines which have been extensively cultured or pas- saged for multiple generations , the degree of retention ( or loss ) of kidney- specific morphology and function is an important issue to consider ... Cell Lines from Rabbit and Pig 417.
Pagina 547
... cell cultures rat renal cell lines derived from , 411-419 . See also LLC - RK1 ; Re- nal cell lines , derived from rabbit and pig kidney isolation of renal cortical cells from , 400 proximal and distal tubular cells from , purification ...
... cell cultures rat renal cell lines derived from , 411-419 . See also LLC - RK1 ; Re- nal cell lines , derived from rabbit and pig kidney isolation of renal cortical cells from , 400 proximal and distal tubular cells from , purification ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml