In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 110
... Cells Jacob N. Finkelstein INTRODUCTION As the lung is a complex parenchymal organ composed of many different cell types , there has always been considerable interest in the development of appro- priate cellular model systems that would ...
... Cells Jacob N. Finkelstein INTRODUCTION As the lung is a complex parenchymal organ composed of many different cell types , there has always been considerable interest in the development of appro- priate cellular model systems that would ...
Pagina 182
... types of mucous cells : surface mucous cells that line the entire surface of ... cell function are quite complex , with many of the chemical mediators ... cell separation techniques circumvent some of the problems that result from the ...
... types of mucous cells : surface mucous cells that line the entire surface of ... cell function are quite complex , with many of the chemical mediators ... cell separation techniques circumvent some of the problems that result from the ...
Pagina 366
... types . Particular nephron seg- ments containing these different cell types cannot be isolated simply by slicing particular portions of the kidney , owing to the convoluted structure of ... Cell Culture Rabbit Cortical Epithelial Cells Taub.
... types . Particular nephron seg- ments containing these different cell types cannot be isolated simply by slicing particular portions of the kidney , owing to the convoluted structure of ... Cell Culture Rabbit Cortical Epithelial Cells Taub.
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml