In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 16
... cerebellar cultures ; cerebral neocortex cultures can be prepared from ani- mals ranging from newborn to 5 postnatal days ; and 14-15 day fetal mice or rats are optimal for spinal cord - DRG cultures . Cerebellum The procedure for ...
... cerebellar cultures ; cerebral neocortex cultures can be prepared from ani- mals ranging from newborn to 5 postnatal days ; and 14-15 day fetal mice or rats are optimal for spinal cord - DRG cultures . Cerebellum The procedure for ...
Pagina 20
... cerebellar cultures . ( A ) Low - power view of a cerebellar explant , 23 DIV , illustrating cortical ( Co ) and deep nucleus ( Nu ) regions . Lamination is evident in cortex on the right - hand side and near the top of the ...
... cerebellar cultures . ( A ) Low - power view of a cerebellar explant , 23 DIV , illustrating cortical ( Co ) and deep nucleus ( Nu ) regions . Lamination is evident in cortex on the right - hand side and near the top of the ...
Pagina 23
... cerebellum . as Distinct from both cerebellar and cerebral neocortex cultures are spinal cord - DRG explants , as illustrated in Fig . 3. A spinal cord explant with DRG at- tached by dorsal roots is shown in Fig . 3A . The silver ...
... cerebellum . as Distinct from both cerebellar and cerebral neocortex cultures are spinal cord - DRG explants , as illustrated in Fig . 3. A spinal cord explant with DRG at- tached by dorsal roots is shown in Fig . 3A . The silver ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml