In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 407
... chemical or a disease process can be studied more easily . Where there are ques- tions of nephron cell heterogeneity and susceptibility to chemical injury , the preparations of isolated PT and DT cells in suspension have been extremely ...
... chemical or a disease process can be studied more easily . Where there are ques- tions of nephron cell heterogeneity and susceptibility to chemical injury , the preparations of isolated PT and DT cells in suspension have been extremely ...
Pagina 505
... chemical . The following discussion describes how to design and implement percutaneous penetration studies depending on the particular situation of interest . MATERIALS AND REAGENTS Test Chemical The physicochemical characteristics of ...
... chemical . The following discussion describes how to design and implement percutaneous penetration studies depending on the particular situation of interest . MATERIALS AND REAGENTS Test Chemical The physicochemical characteristics of ...
Pagina 512
... chemical can be measured in the skin and receptor fluid . Measurement of Percutaneous Penetration Traditionally , measurement of skin penetration has been done by quantifying the accumulation of test chemical in the receptor fluid which ...
... chemical can be measured in the skin and receptor fluid . Measurement of Percutaneous Penetration Traditionally , measurement of skin penetration has been done by quantifying the accumulation of test chemical in the receptor fluid which ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml