In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 28
... containing cells from the RMT with CS cells results in coaggregates ( RMT - CS ) that contain , by mor- phological and neurochemical criteria , a functional nigrostriatal dopaminergic projection albeit on a diminished scale . The DA ...
... containing cells from the RMT with CS cells results in coaggregates ( RMT - CS ) that contain , by mor- phological and neurochemical criteria , a functional nigrostriatal dopaminergic projection albeit on a diminished scale . The DA ...
Pagina 31
... containing neurons ( 18,19 ) . Details of the preparation of coaggregates contain- ing septal and hippocampal neurons are provided by Hsiang et al . ( 12 ) . A visual guide for the dissection is provided in Fig . 2. The forebrain is ...
... containing neurons ( 18,19 ) . Details of the preparation of coaggregates contain- ing septal and hippocampal neurons are provided by Hsiang et al . ( 12 ) . A visual guide for the dissection is provided in Fig . 2. The forebrain is ...
Pagina 369
... containing the kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated kidneys are conve- nient for sterile transport to the tissue culture laboratory without spillage of medium . Each kidney is then placed in ...
... containing the kidneys are kept at 4 ° C throughout the procedure . Tubes containing the isolated kidneys are conve- nient for sterile transport to the tissue culture laboratory without spillage of medium . Each kidney is then placed in ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml