In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 19
... petri dishes lined with black filter paper , a 15 × 100 mm petri dish with processed and ster- ilized square coverslips , an empty 15 × 100 mm petri dish , and an inverted black embryological watch glass ( A. H. Thomas ) . Metal culture ...
... petri dishes lined with black filter paper , a 15 × 100 mm petri dish with processed and ster- ilized square coverslips , an empty 15 × 100 mm petri dish , and an inverted black embryological watch glass ( A. H. Thomas ) . Metal culture ...
Pagina 151
... culture dishes , 2 ml per 35 - mm dish ( No. 25000 , Corning , Corning , NY ) , and placed into a humidified continuous gas flow incubator ( Forma Scientific ) . The incubator is maintained at 37 ° C , and a pH of 7.2 to 7.4 is ...
... culture dishes , 2 ml per 35 - mm dish ( No. 25000 , Corning , Corning , NY ) , and placed into a humidified continuous gas flow incubator ( Forma Scientific ) . The incubator is maintained at 37 ° C , and a pH of 7.2 to 7.4 is ...
Pagina 328
... culture conditions . Variations in culture conditions have been discussed by Braun et al . ( 15,16,24 ) , and only ... dish for a total of 1 × 106 cells per dish . As an alternative to plating directly on plastic , culture dishes may be ...
... culture conditions . Variations in culture conditions have been discussed by Braun et al . ( 15,16,24 ) , and only ... dish for a total of 1 × 106 cells per dish . As an alternative to plating directly on plastic , culture dishes may be ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml