In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 303
... digestion was incomplete . These paremeters can be used to determine optimal enzyme concentration . Some preparation - to - preparation variation in digestion is common and may also be compensated for during the procedure by adjusting ...
... digestion was incomplete . These paremeters can be used to determine optimal enzyme concentration . Some preparation - to - preparation variation in digestion is common and may also be compensated for during the procedure by adjusting ...
Pagina 360
... digestion 15 min -A C 目 40 % 45 % 50 % 556 90 % 2000 CENTRIFUGE 250 X 0 FOR 15 MINUTES 40 % 45 % 50 % 559 % 90 % MINCE TISSUE let aggregates settle for 1 min collagenase digestion 15 min DISTAL TUBULES AND DEBRIS STRAIGHT PROXIMAL ...
... digestion 15 min -A C 目 40 % 45 % 50 % 556 90 % 2000 CENTRIFUGE 250 X 0 FOR 15 MINUTES 40 % 45 % 50 % 559 % 90 % MINCE TISSUE let aggregates settle for 1 min collagenase digestion 15 min DISTAL TUBULES AND DEBRIS STRAIGHT PROXIMAL ...
Pagina 361
... digested tubular segments but containing the cocktail en- zymes ) are then returned to each flask for further digestion of the remaining tis- sue . The soft pellets of harvested tubules are washed once , with 30 ml of ice- cold DME ...
... digested tubular segments but containing the cocktail en- zymes ) are then returned to each flask for further digestion of the remaining tis- sue . The soft pellets of harvested tubules are washed once , with 30 ml of ice- cold DME ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
Parole e frasi comuni
1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml