In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 66
... dissociated cells is transferred to a 15 - ml centrifuge tube containing 7 ml of the enzyme inhibitor solution . Fresh enzyme solution ( the remaining 2 ml of the 5 - ml aliquot ) is added to the contents of the flat - bottomed ...
... dissociated cells is transferred to a 15 - ml centrifuge tube containing 7 ml of the enzyme inhibitor solution . Fresh enzyme solution ( the remaining 2 ml of the 5 - ml aliquot ) is added to the contents of the flat - bottomed ...
Pagina 324
... dissociated cells . 5. Add fresh aliquots of enzyme as above to trypsinizing flask . Repeat incuba- tion as in Step ... dissociate clumps of cells retained on the filter . 8. Combine the four parenchymal cell suspensions . Count a 0.1 ...
... dissociated cells . 5. Add fresh aliquots of enzyme as above to trypsinizing flask . Repeat incuba- tion as in Step ... dissociate clumps of cells retained on the filter . 8. Combine the four parenchymal cell suspensions . Count a 0.1 ...
Pagina 343
... dissociate the remaining tissue , and the pellets may be stored on ice until the next stage of the procedure . If ... dissociated tissue is resuspended in 70 ml of 50 % Percoll and evenly split into two 35 - ml polystyrene Oak Ridge ...
... dissociate the remaining tissue , and the pellets may be stored on ice until the next stage of the procedure . If ... dissociated tissue is resuspended in 70 ml of 50 % Percoll and evenly split into two 35 - ml polystyrene Oak Ridge ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml