In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 66
... dissociation is checked again . At this stage most cells ap- pear dissociated and are transferred to the enzyme inhibitor solution . Remaining tissue clumps are gently broken up by several passages through the mouth of a 5 - ml pipette ...
... dissociation is checked again . At this stage most cells ap- pear dissociated and are transferred to the enzyme inhibitor solution . Remaining tissue clumps are gently broken up by several passages through the mouth of a 5 - ml pipette ...
Pagina 111
... Dissociation of Type II Cells from Lung Several different strategies have been developed to dissociate cells from their at- tachments to neighboring cells as well as the underlying extracellular matrix . These can be grouped into three ...
... Dissociation of Type II Cells from Lung Several different strategies have been developed to dissociate cells from their at- tachments to neighboring cells as well as the underlying extracellular matrix . These can be grouped into three ...
Pagina 343
... dissociation process are the disease state of the kidneys and the collagenase . As discussed above , the use of SPF animals helps to ensure healthy kidneys which provide consistent tubule yields . Because collagenase preparations ...
... dissociation process are the disease state of the kidneys and the collagenase . As discussed above , the use of SPF animals helps to ensure healthy kidneys which provide consistent tubule yields . Because collagenase preparations ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml