In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 66
... enzyme inhibitor solution . Fresh enzyme solution ( the remaining 2 ml of the 5 - ml aliquot ) is added to the contents of the flat - bottomed centrifuge tube , and the tube is returned to the nutator . After 20 min the progress of the ...
... enzyme inhibitor solution . Fresh enzyme solution ( the remaining 2 ml of the 5 - ml aliquot ) is added to the contents of the flat - bottomed centrifuge tube , and the tube is returned to the nutator . After 20 min the progress of the ...
Pagina 112
... enzymes both among and within companies . Most often the commercial enzyme preparations will contain other enzymes in addition to those specified . It is well known that crude trypsin con- tains elastase , chymotrypsin , amylase ...
... enzymes both among and within companies . Most often the commercial enzyme preparations will contain other enzymes in addition to those specified . It is well known that crude trypsin con- tains elastase , chymotrypsin , amylase ...
Pagina 323
... enzyme as above to trypsinizing flask . Repeat incuba- tion as in Step 2 . 5. Add 25 ml MEM with 10 % calf serum to filtered cell suspension on ice to neutralize enzyme activity . Centrifuge at 300 g and 4 ° C , for 10 min . Aspirate ...
... enzyme as above to trypsinizing flask . Repeat incuba- tion as in Step 2 . 5. Add 25 ml MEM with 10 % calf serum to filtered cell suspension on ice to neutralize enzyme activity . Centrifuge at 300 g and 4 ° C , for 10 min . Aspirate ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml