In Vitro Biological SystemsCharles A. Tyson, John M. Frazier Academic Press, 1993 - 568 pagine Aims to provide researchers with basic techniques employed by widely-recognized scientists in preparing and maintaining the biological components of in vitro model systems. The methods have been organized by organ systems for easy reference. |
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Pagina 437
... Figure 4 The tubules are allowed to settle to the. Figure 1 Tubules are minced into pieces of approximately 1-2 mm2 using a sterile sin- gle - edged razor blade . Figure 3 The tubule - medium mixture is drawn up. 39. Sertoli - Germ Cell ...
... Figure 4 The tubules are allowed to settle to the. Figure 1 Tubules are minced into pieces of approximately 1-2 mm2 using a sterile sin- gle - edged razor blade . Figure 3 The tubule - medium mixture is drawn up. 39. Sertoli - Germ Cell ...
Pagina 440
... Figure 7 The collagenase digestion breaks down the tubules into. Figure 6 The trypsinization step dissociates tubules into long cordlike structures when examined microscopically . Bar : 200 μm . Figure 8 Appearance of tubule - germ cell ...
... Figure 7 The collagenase digestion breaks down the tubules into. Figure 6 The trypsinization step dissociates tubules into long cordlike structures when examined microscopically . Bar : 200 μm . Figure 8 Appearance of tubule - germ cell ...
Pagina 519
... Figure 3 Glucose utilization ( mean ± SD ) in a series of 212 normal skin flaps . D E Figure 4 Light micrograph demonstrating viable skin after 8 hr of perfusion in an IPPSF preparation . E , Epidermis ; D , dermis . Hematoxylin and ...
... Figure 3 Glucose utilization ( mean ± SD ) in a series of 212 normal skin flaps . D E Figure 4 Light micrograph demonstrating viable skin after 8 hr of perfusion in an IPPSF preparation . E , Epidermis ; D , dermis . Hematoxylin and ...
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In Vitro Biological Systems: Methods in Toxicology, Volume 1 Charles A. Tyson,John M. Frazier Anteprima limitata - 2016 |
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1993 by Academic Academic Press acid aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula catecholamine cell cultures cell suspension cellular centrifuge tube chemical chromaffin cells Clara cells collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation endothelial cells enzyme epithelial cells explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth growth medium hepatocytes HEPES incubated inhibits isolated kidneys Kupffer cells laboratory layer lipocytes liver macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurochemical neurons obtained Pasteur pipette pellet Percoll perfusion petri dish Pharmacol pipette plastic plates preparation procedure protein proximal tubule rabbit REAGENTS reaggregates removed renal resuspended scissors segments Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells vessel viability vitro vivo Volume 1A Copyright washed µg/ml